Day: May 24, 2024

46. ํ‹€ / the norm

๋น„๊ฐ€ ์™€๋„ ์šฐ์‚ฐ์„ ์“ฐ์ง€ ๋ง์•„ ๋ด์š”

์ž๋ฆฌ๊ฐ€ ๋น„์–ด๋„ ์•‰์ง€ ๋ง์•„ ๋ด์š”

๊ฐ€์งˆ์ˆ˜ ์žˆ๋Š” ๊ฑธ ๋†“์ณ๋„ ๋ณด์•„์š”

์ƒ๊ฐ์˜ ํ‹€์—์„œ ๋ฒ—์–ด๋‚˜ ๋ด์š”

์ด๊ฒƒ๋„ ์ €๊ฒƒ๋„ ์ฆ๊ฒจ๋ด์š”

Even if it rains, don’t use an umbrella

Even if the seat is empty, don’t sit down.

Even if you have what you could have, Just miss it out  

Step outside the norm

Enjoy this and that 

Bioinformatics: Featurecount Install and more

Subhead-2.0.2

https://github.com/ShiLab-Bioinformatics/subread/releases

  1. wget https://github.com/ShiLab-Bioinformatics/subread/releases/download/2.0.2/subread-2.0.2-macOS-x86_64.tar.gz
  2. tar -xvf subread-2.0.2-macOS-x86_64.tar.gz
  3. mv -v subread-2.0.2-macOS-x86_64 apps/
  4. nano .zshrc
  5. add: Export PATH=$PATH:/Users/__/___/subread/bin
  6. get gif or make gif: https://useast.ensembl.org/Mus_musculus/Info/Index
  7. use eg) featureCounts -T 5 -a annotation-subread/mouse_ensembl/Mus_musculus.GRCm38.102.gtf  -o quants/KO_count_matrix.txt HISAT2/SRR2936859.bam HISAT2/SRR2936860.bam HISAT2/SRR2936861.bam HISAT2/SRR2936864.bam HISAT2/SRR2936865.bam
  8. Cat file | less
  9. Cat file | cut f1,7-10> new file (could rename of column in excel!!!)

reference:

https://rdrr.io/bioc/Rsubread/man/buildindex.html

https://github.com/ShiLab-Bioinformatics/subread/releases

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