I just have courage to take part in local author fair in Carlsbad, CA
Some more about this event
Month: May 2025
Big mountain 🏔️
May 12, 2021, a text message from retired Prof Kim, Hyun-Taek, department of Biological Psychology, Korea University
심리학과 김현택 교수님, 아마도 그 해, 혹은 그 전에 은퇴하셨던 것 같다. 몇몇 은사님들께서 주신 메시지가 컴퓨터에 저장되어 있는데, 이건 그 중 하나이다.
태산에 걸려 넘어지는 일은 없고
돌부리에 걸려 넘어진다고하니
오늘에 집중해서 살게
As people say that we don't stumble over a big mountain,
but we stumble over a stone,
so focus on living TODAY.
😋
향기의 여행, 차강석 | A journey of fragrance, by Mr. Cha
2025년 4월 23일 | April 23, 2025
향기의 여행
살포시 다가온 봄
라일락은 꽃을 피우고
향기를 풍매화처럼 흩뿌린다
향기는 신기루 같은 바람을 타고
정주행이 목적인 시간을 역주행하여
삼라만상(森羅萬象)을 카오스 상태로 만든다
다시 향기는 시간의 정주행을 만들고
향기는 시간의 정주행을 타고 역사를 쓰며
활화산처럼 활기차고 열정 넘치는 젊은 나를 본다
A journey of fragrance
Spring that comes gently,
makes Lilacs bloom and scatter their fragrance
like wind-blown flowers
The Lilacs' fragrance rides the mirage-like wind
and reverses the time that is meant to flow to the future
and turns all things in the universe into chaotic state
The fragrance move a regular journey of time at this time
and rides the journey of time in the future direction, and writes history.
I see my young self,
full of energy and passion,
like an active volcano.
존재 | Existence
2021년 5월 11일, 김수영 | May 11, 2021 by Kim, Sooyoung
길을 걷는다
문득 발 아래 개미를 인식한다
내가
존재하고
내가
일 하는 방식이
나도 모르는 사이
죽어나가는 개미를
만들어 낸다면
나도 열심히
거인들의 발아래에서
죽지 않기 위해
피해다니는 형국이라면
때론
이것이 사는 것이라면
내 발아래 개미를 깨닫는 순간도
내 위 거인의 발바닥을 느끼는 순간도
그닥 유쾌한 경험은 아니다
Walking down the street
Suddenly I realize the ants under my feet
If my existence and the way I work
creates ants that die without me knowing
If I also work hard
to avoid dying under the feet of giants
If sometimes this is living
The moment I realize the ants under my feet and also
the moment I feel the soles of the giants' feet above me
are not very pleasant experiences
Comments
Cabbage looper | Owlet Moths | Trichoplusia ni
May 22, 2025 by Sooyoung
Today, I searched for Trichoplusia ni (cabbage looper) and the reason why it is used for protein expression.
The cabbage looper is a medium-sized moth commonly called owlet moth, and it is known for its distinctive looping movement as a caterpillar. So, the caterpillar is commonly called a cabbage looper, and the adult cabbage loopers are called owlet moths.
A picture of owlet moth from webpage, russellipm.com
You may visit YouTube to see Looper. 🐛
Why it is used for protein expression and how the technology has been developed:
Trichoplusia ni (cabbage looper) is used for protein expression due to its ability to produce recombinant proteins at high levels and with good quality, particularly for SECRETED proteins. Its insect cell lines, such as Tni-FNL, have demonstrated superior protein production compared to other winged insect (lepidopteran) cell lines of Spodoptera frugiperda (fall armyworm moth). Furthermore, some Trichoplusia ni cell lines (Tni-FNL) have shown improved growth rates and the ability to grow at lower temperatures.
Who developed:
The High Five (BTI-Tn-5B1-4) cell line, derived from the cabbage looper (Trichoplusia ni) eggs, was first developed by the Boyce Thompson Institute for Plant Research in 1970. Another Trichoplusia ni cell line, Tni-FNL, was developed by researchers at the National Cancer Institute in 2018.
What we think about protein expression:
Protein yield, Scale-up, Toxicity, Post-translational modification, growth characteristics, how could be advanced further…..
Further reading materials and original references:
- W.F.HINK, 1970, Established Insect Cell Line from the Cabbage Looper, Trichoplusia ni
- Fengrui Zhang, 2008, A New Trichoplusia ni cell line for membrane protein expression using a baculovirus expression vector system
- Esposito Dominic, 2018, Tni-FNL: An Improved Trichoplusia Ni Cell Line for Protein Expression
민들레와 이별, 차강석 | Parting with Dandelion, by Mr. Cha
민들레와 이별
4/18/2025
바람이 쌀랑쌀랑 부는 봄
민들레는 공기가 가득 찬
풍선처럼 공중부양을 한다
하늘로 떠오른 민들레는
산불을 진화하는 헬기같이
홀씨들을 벚꽃처럼 흩뿌린다
홀씨들을 세상으로 떠나보낸 민들레는
아기를 막 낳은 임산부의 마음이 되었다
Parting with Dandelion
4/18/2025
Spring, when the wind blows gently,
floats dandelion into the air,
like a balloon full of air
Dandelion that has risen to the sky
like a helicopter that puts out a forest fire,
scatters its seeds like falling cherry blossom pedals
The dandlion that has sent seeds out in the world,
has the heart of a pregnant woman who has just given birth
What is life?
by Moon family May 11, 2025
On the way home from school, Grace and I discussed what life is. First, Grace said, life needs WATER. Dried things have no life in there, and we discussed eating, pooping and peeing – METABOLISM. Life needs metabolism for ENERGY.
The discussion has been continued, and today during the mother’s day dinner time, we added TEMPERATURE. Corpses have no body temperature she said. Finally, her dad, my hus added SPIRIT, and then,
What else? Grace tried to add OXYGEN, but we did not agree on it, because humans need OXYGEN, but not for all living things.
On this question of “What is life”, I felt that it is very interesting. We could think of the simple question in the view of everything from tiny unicells such as bacteria to the bigger organisms like humans and even the universe. I thought about himan vital signs such as breathing and blood pressure as well.
Suddenly, i murmured the unscientific idea that all things have life because they have birth and extinction.
We might continue thinking of what life is.
Life and Biology stories with Sooyoung and Others 
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