Author: Gungil

존재 | Existence

2021년 5월 11일, 김수영 | May 11, 2021 by Kim, Sooyoung

길을 걷는다
문득 발  아래 개미를 인식한다
 
내가
존재하고
내가
일 하는 방식이
나도 모르는 사이
죽어나가는 개미를
만들어 낸다면
 
나도 열심히
거인들의 발아래에서
죽지 않기 위해
피해다니는 형국이라면
 
때론
이것이 사는 것이라면
 
내 발아래 개미를 깨닫는 순간도
내 위 거인의 발바닥을 느끼는 순간도
그닥 유쾌한 경험은 아니다
Walking down the street
Suddenly I realize the ants under my feet

If my existence and the way I work
creates ants that die without me knowing

If I also work hard
to avoid dying under the feet of giants

If sometimes this is living

The moment I realize the ants under my feet and also 
the moment I feel the soles of the giants' feet above me
are not very pleasant experiences
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Cabbage looper | Owlet Moths | Trichoplusia ni

May 22, 2025 by Sooyoung

Today, I searched for Trichoplusia ni (cabbage looper) and the reason why it is used for protein expression.

The cabbage looper is a medium-sized moth commonly called owlet moth, and it is known for its distinctive looping movement as a caterpillar. So, the caterpillar is commonly called a cabbage looper, and the adult cabbage loopers are called owlet moths.

A picture of owlet moth from webpage, russellipm.com

You may visit YouTube to see Looper. 🐛

Why it is used for protein expression and how the technology has been developed:

Trichoplusia ni (cabbage looper) is used for protein expression due to its ability to produce recombinant proteins at high levels and with good quality, particularly for SECRETED proteins. Its insect cell lines, such as Tni-FNL, have demonstrated superior protein production compared to other winged insect (lepidopteran) cell lines of Spodoptera frugiperda (fall armyworm moth). Furthermore, some Trichoplusia ni cell lines (Tni-FNL) have shown improved growth rates and the ability to grow at lower temperatures. 

Who developed:

The High Five (BTI-Tn-5B1-4) cell line, derived from the cabbage looper (Trichoplusia ni) eggs, was first developed by the Boyce Thompson Institute for Plant Research in 1970. Another Trichoplusia ni cell line, Tni-FNL, was developed by researchers at the National Cancer Institute in 2018.

What we think about protein expression:

Protein yield, Scale-up, Toxicity, Post-translational modification, growth characteristics, how could be advanced further…..

Further reading materials and original references:

민들레와 이별, 차강석 | Parting with Dandelion, by Mr. Cha

민들레와 이별

4/18/2025

바람이 쌀랑쌀랑 부는 봄 
민들레는 공기가 가득 찬
풍선처럼 공중부양을 한다

하늘로 떠오른 민들레는
산불을 진화하는 헬기같이
홀씨들을 벚꽃처럼 흩뿌린다

홀씨들을 세상으로 떠나보낸 민들레는
아기를 막 낳은 임산부의 마음이 되었다
Parting with Dandelion

4/18/2025

Spring, when the wind blows gently,
floats dandelion into the air, 
like a balloon full of air

Dandelion that has risen to the sky
like a helicopter that puts out a forest fire,
scatters its seeds like falling cherry blossom pedals

The dandlion that has sent seeds out in the world, 
has the heart of a pregnant woman who has just given birth

About author Mr. Cha

What is life?

by Moon family May 11, 2025

On the way home from school, Grace and I discussed what life is. First, Grace said, life needs WATER. Dried things have no life in there, and we discussed eating, pooping and peeing – METABOLISM. Life needs metabolism for ENERGY. 

The discussion has been continued, and today during the mother’s day dinner time, we added TEMPERATURE. Corpses have no body temperature she said. Finally, her dad, my hus added SPIRIT, and then,

What else? Grace tried to add OXYGEN, but we did not agree on it, because humans need OXYGEN, but not for all living things.  

On this question of “What is life”, I felt that it is very interesting. We could think of the simple question in the view of everything from tiny unicells such as bacteria to the bigger organisms like humans and even the universe. I thought about himan vital signs such as breathing and blood pressure as well.

Suddenly, i murmured the unscientific idea that all things have life because they have birth and extinction. 

We might continue thinking of what life is.

주인, Ownership

주인
2021년 4월30일

출근을 위해서
새벽  4:30 분 혹은 5:30분 집을 나선다
 
지난
겨우내 눈이 많이 왔다
눈이 올때 마다 1시간씩 더 일찍 읽어났다
 
눈을 치웠다
마당 집 앞까지 길을 냈다
나는 나서지만
들어오셔야 하는
딸아이의 아침선생님을 위함이기도 했다
이층 분들을 위함이기도 했다
 
눈을 치우며
생각했다
 
대표님을
 
회사도 주인의식을 가진 사람들이
배려를 하며 이끄는 것이라고
 
주인의식을 가지고 눈은 치우고 길을 냈지만
난 집주인은 아니다 그저 잠시 머문 식구
 
주인의식도 전파되는듯하다
Ownership
April 30, 2021

To go to work
I leave the house 
at 4:30 or 5:30 am in the morning.

Through the winter
It snowed a lot
I woke up an hour earlier 
every time it snowed

I cleared the snow
I made a path through the yard to the front of the house
I went out, but
it was for my daughter's morning teacher who had to come in
and for the people on the units of the second floor

While clearing the snow
I thought about

Our CEO and

that the company should be led by peoples with a sense of ownership and thoughtful caring

I cleared the snow and made a path with a sense of ownership
but I'm not the owner of the house.

I'm just a member who stayed for a while
It seems like the sense of ownership is spreading with the leadership

봄비, 차강석 | Spring rain, by Mr. Cha

   봄비
   2025년 5월 2일, 차강석 씀

   봄비가 부슬부슬 내리고
   꽃잎들은 바닥도 모르도록 떨어진다

   봄이 살며시 다가와서
   사람들과 꽃잎들 또 온갖 생물들이 활기를 띈다

   사랑하는 것들은 너무 빨리 이별을 고하듯
   광대 얼굴처럼 어여쁜 봄도 의식도 할 수 없이 가 버린다
Spring Rain
May 2, 2025, by Cha

Spring rain is falling gently
Flower petals scatter without even noticing the ground

Spring comes gently and
All creatures including people and flowers liven up.

As if things we love leave too soon
The beautiful spring, like a clown's face, goes away without us even realizing it

About author Mr. Cha

Zen of Python

By Tim Peters

You might see this, when open terminal of your computer, and write python

>>>import this

The Zen of Python, by Tim Peters

Beautiful is better than ugly.
Explicit is better than implicit.
Simple is better than complex.
Complex is better than complicated.
Flat is better than nested.
Sparse is better than dense.
Readability counts.
Special cases aren't special enough to break the rules.
Although practicality beats purity.
Errors should never pass silently.
Unless explicitly silenced.
In the face of ambiguity, refuse the temptation to guess.
There should be one-- and preferably only one --obvious way to do it.
Although that way may not be obvious at first unless you're Dutch.
Now is better than never.
Although never is often better than *right* now.
If the implementation is hard to explain, it's a bad idea.
If the implementation is easy to explain, it may be a good idea.
Namespaces are one honking great idea -- let's do more of those!

visit PEP8 guide

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