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Tag: sooyoung
존재 | Existence
2021년 5월 11일, 김수영 | May 11, 2021 by Kim, Sooyoung
길을 걷는다
문득 발 아래 개미를 인식한다
내가
존재하고
내가
일 하는 방식이
나도 모르는 사이
죽어나가는 개미를
만들어 낸다면
나도 열심히
거인들의 발아래에서
죽지 않기 위해
피해다니는 형국이라면
때론
이것이 사는 것이라면
내 발아래 개미를 깨닫는 순간도
내 위 거인의 발바닥을 느끼는 순간도
그닥 유쾌한 경험은 아니다
Walking down the street
Suddenly I realize the ants under my feet
If my existence and the way I work
creates ants that die without me knowing
If I also work hard
to avoid dying under the feet of giants
If sometimes this is living
The moment I realize the ants under my feet and also
the moment I feel the soles of the giants' feet above me
are not very pleasant experiences
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Cabbage looper | Owlet Moths | Trichoplusia ni
May 22, 2025 by Sooyoung
Today, I searched for Trichoplusia ni (cabbage looper) and the reason why it is used for protein expression.
The cabbage looper is a medium-sized moth commonly called owlet moth, and it is known for its distinctive looping movement as a caterpillar. So, the caterpillar is commonly called a cabbage looper, and the adult cabbage loopers are called owlet moths.
A picture of owlet moth from webpage, russellipm.com
You may visit YouTube to see Looper. 🐛
Why it is used for protein expression and how the technology has been developed:
Trichoplusia ni (cabbage looper) is used for protein expression due to its ability to produce recombinant proteins at high levels and with good quality, particularly for SECRETED proteins. Its insect cell lines, such as Tni-FNL, have demonstrated superior protein production compared to other winged insect (lepidopteran) cell lines of Spodoptera frugiperda (fall armyworm moth). Furthermore, some Trichoplusia ni cell lines (Tni-FNL) have shown improved growth rates and the ability to grow at lower temperatures.
Who developed:
The High Five (BTI-Tn-5B1-4) cell line, derived from the cabbage looper (Trichoplusia ni) eggs, was first developed by the Boyce Thompson Institute for Plant Research in 1970. Another Trichoplusia ni cell line, Tni-FNL, was developed by researchers at the National Cancer Institute in 2018.
What we think about protein expression:
Protein yield, Scale-up, Toxicity, Post-translational modification, growth characteristics, how could be advanced further…..
Further reading materials and original references:
- W.F.HINK, 1970, Established Insect Cell Line from the Cabbage Looper, Trichoplusia ni
- Fengrui Zhang, 2008, A New Trichoplusia ni cell line for membrane protein expression using a baculovirus expression vector system
- Esposito Dominic, 2018, Tni-FNL: An Improved Trichoplusia Ni Cell Line for Protein Expression
What is life?
by Moon family May 11, 2025
On the way home from school, Grace and I discussed what life is. First, Grace said, life needs WATER. Dried things have no life in there, and we discussed eating, pooping and peeing – METABOLISM. Life needs metabolism for ENERGY.
The discussion has been continued, and today during the mother’s day dinner time, we added TEMPERATURE. Corpses have no body temperature she said. Finally, her dad, my hus added SPIRIT, and then,
What else? Grace tried to add OXYGEN, but we did not agree on it, because humans need OXYGEN, but not for all living things.
On this question of “What is life”, I felt that it is very interesting. We could think of the simple question in the view of everything from tiny unicells such as bacteria to the bigger organisms like humans and even the universe. I thought about himan vital signs such as breathing and blood pressure as well.
Suddenly, i murmured the unscientific idea that all things have life because they have birth and extinction.
We might continue thinking of what life is.
주인, Ownership
주인
2021년 4월30일
출근을 위해서
새벽 4:30 분 혹은 5:30분 집을 나선다
지난
겨우내 눈이 많이 왔다
눈이 올때 마다 1시간씩 더 일찍 읽어났다
눈을 치웠다
마당 집 앞까지 길을 냈다
나는 나서지만
들어오셔야 하는
딸아이의 아침선생님을 위함이기도 했다
이층 분들을 위함이기도 했다
눈을 치우며
생각했다
대표님을
회사도 주인의식을 가진 사람들이
배려를 하며 이끄는 것이라고
주인의식을 가지고 눈은 치우고 길을 냈지만
난 집주인은 아니다 그저 잠시 머문 식구
주인의식도 전파되는듯하다
Ownership
April 30, 2021
To go to work
I leave the house
at 4:30 or 5:30 am in the morning.
Through the winter
It snowed a lot
I woke up an hour earlier
every time it snowed
I cleared the snow
I made a path through the yard to the front of the house
I went out, but
it was for my daughter's morning teacher who had to come in
and for the people on the units of the second floor
While clearing the snow
I thought about
Our CEO and
that the company should be led by peoples with a sense of ownership and thoughtful caring
I cleared the snow and made a path with a sense of ownership
but I'm not the owner of the house.
I'm just a member who stayed for a while
It seems like the sense of ownership is spreading with the leadership
가혹한 시간 | Ruthless time
2021년 1월 22일 | Jan 22, 2021
가옥하고 잔인한 시간이다.
아이가 운다.
난 회사에서 시간을 채워야 한다.
의미 없는 시간이다.
빨리 끝내고 나갈수 있는 시간이다.
아이는 운다.
이것이 맡닥들이는 현실이다.
견디어내자.
딸 사랑한다. 같이 견디어 내 보자. 한번 해보자.
출근시간 퇴근시간 지켜보자
---------
유아 단축근무는 필요하다
It is ruthless killing time.
A child cries.
I have to fill the working time.
It's meaningless killing time.
It's time to finish quickly and leave.
The child cries.
It is a daily routine that working moms face.
Let's endure it.
Love child. Let's endure it together. Let's try it.
I keep the start and end times to work.
---------
It is necessary that parents could have flexible working hours for kids
디지털화 | Digitalization
김수영 2020년 11월 19일 | Sooyoung Kim, Nov 19 2020
디지털화
줄어드는 인구
가속화되는 디지털화
학교도 기계속으로
은행도 기계안으로
친구도 기계속으로
거래도 기계안으로
분명 세상이 엄청 변하고 있는것이다
Digitalization
Dwindling population
Accelerating digitalization
Schools are virtual
Banks are also virtual
Friends are also virtual
Transactions and communications are also virtual
Dramatically changing world, we are in.
Life and Biology stories with Sooyoung and Others 
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